The PHD ULTRA™ CP Syringe Pump is the first of its kind to allow operation under constant pressure or constant flow. Historically, the only means available to dispense at constant pressure has involved the use of various amplifiers and other accessories/software. The PHD ULTRA™ CP Syringe Pump, when combined with virtually any commercially available pressure transducer/amplifier combination with 0-10V DC analog output, results in a constant pressure dispensing system. This sytem can deliver fluids with an applied force up to 1,000 lbs (depending upon the pump).
Using Harvard Apparatus syringe pump technology and software-controlled pressure monitoring, the PHD ULTRA™ CP Syringe Pump is able to maintain a user-defined system pressure ±2%. A user-adjustable sensitivity setting allows for the customization of the system response time necessary to attain the set pressure. The PHD ULTRA™ CP Syringe Pump allows the user to set pressures in the units of their choice including mmHg, kPa, and psi.

PHD ULTRA™ CP 4400 Syringe Pump Infuse/Withdraw Programmable

The PHD ULTRA™ CP Syringe Pump is the first of its kind to allow operation under constant pressure or constant flow. Historically, the only means available to dispense at constant pressure has involved the use of various amplifiers and other accessories/software. The PHD ULTRA™ CP, when combined with virtually any commercially available pressure transducer/amplifier combination with 0-10V DC analog output, results in a constant pressure dispensing system. This sytem can deliver fluids with an applied force up to 1,000 lbs (depending upon the pump).

Features:

• Enables continuous pressure-controlled infusion
• Use in constant flow or constant pressure mode
• Enhances safety for sensitive infusion targets and physiological experimentation
• Compatible with a wide range of pressure transducers
• Automatically adjusts flow rate to maintain constant pressure
• Alpha/numeric keyboard without a PC
• Icon operation
• New color LCD touch screen
• Up-front control knobs for ease of operation
• Vertical or horizontal orientation
• Adjustable linear force to 200 lbs across the entire flow range
• CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme Approved
• 2-year warranty

Applications:

• Short-term organ perfusion studies that require the maintenance of physiologic conditions.
• Introduction of chemical reactants in a controlled manner.
• Ocular injections and perfusions.
• Small animal whole-body perfusions.
• Constant pressure microfluidic mixing.
• Administration of genetic material into organs without viral vectors.

Superior Functionality

Using Harvard Apparatus syringe pump technology and software-controlled pressure monitoring, the PHD ULTRA™ CP Syringe Pump is able to maintain a user-defined system pressure ±2%.

A user-adjustable sensitivity setting allows for the customization of the system response time necessary to attain the set pressure. The PHD ULTRA™ CP Syringe Pump allows the user to set pressures in the units of their choice including mmHg, kPa, and psi.

Essential Application Tools
The PHD ULTRA™ CP Syringe Pump can work with any pressure transducer and amplifier that will output a 0-10V or similar signal. We offer a wide variety of pressure transducers and amplifiers to suit your particular application. Our most popular physiological pressure transducer is our APT-300, a fluid-filled unit with a pressure range of ±300mmHg and a sensitivity of 5µV/V/mmHg (±1%). This has an easily replaceable transducer head. For low pressure applications, such as maintaining the pressure in an eye, we have the P75 Venous Pressure Transducer. This pressure transducer has a pressure range of ñ75mmHg and a sensitivity of 1mV/mmHg. When the customer requires a separate amplifier, the unit of choice is the model 601 Transducer AmplifierModule, or TAM-A. As a PLUGSYS module, this DC Bridge Amplifier requires the use of a PLUGSYS housing with a power supply. Cases holding 2, 5, or 10 modules are available.

Pressure & Flow Rate Data
Data can be monitored via RS-232 from the PHD ULTRA™ CP Syringe Pump to a PC. In practice, the 0-10V analog output of a pressure transducer amplifier is connected to the analog input on the rear panel of the pump. The amplifier or signal conditioner can be provided by the customer, or accomplished with various tranducers and amplifiers available from Harvard Apparatus. The pressure range may be scaled to fit the available transducer voltage output for systems that output less than 10 V. While in constant pressure mode, in addition to the set and actual pressure, the pump displays the flow rate. This data may be output for further analysis with a variety of data acquisition packages.

Accuracy & Reproducibility
In addition to constant pressure mode, the PHD ULTRA™ CP may also be used in flow mode with its worldrenowned accuracy and reproducibility.
All PHD ULTRA™ CP Syringe Pumps are infuse/withdraw programmable models. When used in flow mode, these offer programmable features such as method storage and flow programming functions to allow the user to create simple to complex methods. The PHD ULTRA™ CP Syringe Pump is available in a variety of configurations to suit the desired pressure or flow rate ranges.

Advanced Programming Features:
• Flow Programming – Change the flow with time, volume or a triggered event as many times as you like
• Bolus – Inject a drug (or drugs) in a high quantity at once. The bolus injection can be made in time or volume.
• Concentration Delivery – Calibrate flow in concentration units of mg/kg easily so flow is calibrated to concentration of drug and animal weight.
• Gradients
• % ratio – up to three solvents
• I/O — dedicated and user defined I/O
• Pulsed Flow- so you can program the pulse easily

Widest Flow Rate Range
This pump is engineered to provide flow accuracy within 0.35% and reproducibility within 0.05%. One syringe from 0.5 µl to 140 ml pumps at a range of 3.06 pl/min to 215.8 ml/min.

Maximum Experimental Versatility
The pump can be oriented vertically or horizontally for optimum experimental connectivity. This pump comes standard to hold 1 syringe.

Easy-to-Use Interface
The PHD ULTRA™ color LCD touch screen graphic interface is divided into three basic areas: Operations Display, Message Area, and Navigation. This configuration allows you to easily move through all menu selections and data entry by gently touching the onscreen buttons with a finger or the tip of a soft, non-sharp object such as a pencil eraser.

The Methods Main or Quick Start screens are the primary ?home? for the applications. From those screens you access all the commands needed to operate the PHD ULTRA™ as well as the main system settings.

The Message Area of the touch screen is used to display helpful instructions for the currently displayed screen. It is also used to display error or warning messages to indicate problem conditions in a Method or error conditions during pump operation.

The software is organized into three main Navigational branches, the quick start operations, preloaded/user-defined Methods, and systems settings. You can control operations directly with the touch screen or remotely from an independent computer or device via the external I/O interface.

Program Description
To operate the PHD ULTRA™ the user defines all the required parameters for infusing or withdrawing liquids through a Pump Control Method. The basic operation is a simple 4-step procedure:

1. Select a method
2. Enter operating parameters
3. Preview your method
4. Run your method

Applications
• Animal Infusions or Withdrawals – The PHD ULTRA™ will control the delivery of varying % of nutrients or drugs infused into animals, flush lines using catheters, needles, cannulae or microdialysis.
• Proportioning and Delivering of Mixtures – Mixing gradients or proportions with independent control of two liquids.
• Aerosol for Coating – The pump at high pressure can create an aerosol for the delivery of coating materials such as pharmaceutical tablets and aerosol studies.
• Delivery to Mass Spectroscopy – The delivery of fluids to the MS for calibration, matrix addition or ESI sample.
• Compensating Flows – The continuous infusion and simultaneous withdrawal of liquids for cell cultures or perfusion chambers.
• Dispensers/Injectors — Adhesives, Cell injection, MRI Dyes, Activators/Enzymes, Flow injection, Microreaction vessels, or Stereotaxic delivery.

Advanced GLP Documentation Features:
• Experiment parameter download information to PC
• Alpha/numeric capability

Accessories
A full range of accessories are compatible with the PHD ULTRA™ including syringe heaters, nanofluidic circuits, connectors, tubing, syringes and more.

Specifications	88-3016
Classification	Class I
Dimensions L x D x H in (cm)	12.0 x 8.5 x 7.25 (30.48 x 21.59 x 18.4)
Display	4.3″ WQVGA TFT Color Display with Touchpad
Drive Motor	1.8° Stepper Motor
Environmental Humidity	20% to 80% RH, non condensing
Environmental Operating Temperature English	40°F to 104°F
Environmental Operating Temperature Metric	4°C to 40°C
Environmental Storage Temperatue English	14°F to 158°F
Environmental Storage Temperatue Metric	-10°C to 70°C
Flow Accuracy	±0.35%
Flow Rate Maximum	215.8 ml/min using 140 ml syringe
Flow Rate Minimum	3.06 pl/min using 0.5 µl syringe
I O TTL Connectors	15 pin D-Sub Connector
Input Power	50 W, 0.5 A fuse
Installation Category	II
Max Linear Force	200 lbs @ 100% Force Selection
Mode of Operation	Continuous
Motor Drive Control	Microprocessor with 1/16 microstepping
Net Weight English	12.1 lb
Net Weight Metric	5.5 kg
No of Syringes	1
Non Volatile Memory	Storage of all settings
Number of Microsteps per one rev of Lead Screw	6,400
Pollution Degree	1
Pressure Accuracy	±2%
Pump Configuration	Standard
Pump Function	Infuse/Withdraw Programmable
Pusher Travel Rate Maximum	190.8 mm/min
Pusher Travel Rate Minimum	0.36 µm/min
RS 232 Connectors	9 pin D-Sub Connector
Regulatory Certifications	CE, ETL, (UL, CSA), WEEE, EU RoHS & CB Scheme
Step Rate Maximum	52 µsec/µstep
Step Rate Minimum	27.5 sec/µstep
Syringe Rack Type	Standard Rack
Syringe Size Maximum	140 ml
Syringe Size Minimum	0.5 µl
USB Connectors	Type B
Voltage Range	100-240 VAC, 50/60 Hz
Classification	Class I

Minhee K. Ko, Aleksandr Yelenskiy, Jose M. Gonzalez & James C.H. Tan (2014 ) Feedback-controlled constant-pressure anterior chamber perfusion in live mice Molecular Vision